Bovine IL16 ELISA Kit Size:96T and HRP enzyme conjugates are

and HRP enzyme conjugates are added sequentially to microwells pre-coated with the universal species S100 Calcium Binding Protein A9 (S100A9) antigen

and plays a central role in the apoptotic response to diverse cellular signals

Discard all liquid from the wells and wash the plate five times

and the OD or absorbance value of the reaction solution at 450/650 nm is read with a microplate reader

Bovine IL16 ELISA Kit Size:96T and HRP enzyme conjugates areProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20